l68 Oscar Riddle 



and muscles. The futile attempts of Hofbauer, Sitowski and 

 Whitehead to determine the form in which fats are absorbed from 

 the alimentary tract have also been mentioned. Franz and von 

 Stejskal ('02) made extended studies on the fat metabolism of a chy- 

 luricbymeansofthestain. When stained fat was fed to the patient 

 the colored fat appeared in the urine in four hours and continued 

 always less than twenty-four hours. When, however, stained fat 

 was injected subcutaneously into the shoulder it did not appear in 

 the urine. 



Sitowski with this stain undertook the solution of certain prob- 

 lems of digestion in the caterpillar of certain moths. He made little 

 progress with these problems, but in the course of his investiga- 

 tions discovered a deposit of the dye in the eggs (primary oocytes) 

 of these forms. The writer has used Sudan in a number of studies 

 and for several purposes. He will mention at this point merely 

 its use in determining the time and rate of growth of the eggs 

 (primary oocytes) of fowls and turtles; in the study of some spec- 

 ial features of fat metabolism; and to gain some information as to 

 the inter-relation of the soma and germ cells, including transmis- 

 sion and inheritance behavior. Gage has followed the first report 

 of our results with further studies on the behavior and distribu- 

 tion of this dye in the developing fowl, its appearance in the milk, 

 and with negative results, its passage through the placenta. 



(4) Sudan III and Other Pigments m Inheritance 



The only recorded cases known to the writer, of the de- 

 posit and persistance of foreign or maternal pigments within 

 germ cells, are the following: Schmidt ('91) found that Alkanna- 

 colored fat was taken up by apparentlv all plant cells; the ovules 

 of these plants are not specifically mentioned as obtaining part 

 of this coloring matter.'^ Pizon ('01) states (p. 170) that ''the first 

 pigment of the larva (Botrvllides) proceeds from the maternal 

 organism by migration. " His observations are not conclusive. 

 Sitowski ('05) fed Sudan to caterpillars and obtained the stain 

 within their eggs. He now reports ('09) the presence of the stain 



•' Fat colored with Alcaniiin had been used by Pfeffer (Osmotische Untersuchuiigen, 1877) to 

 color the fat being injested by Myxomycete plasmodia. 



