Sudan III in Metabol/sm and Inheritance 1 71 



showed the characteristic color of Sudan at their peripheries. The 

 thin, folhcular membranes were sHghtly tinged with red. In 

 January four similar turtles were fed and killed in the same manner. 

 In none of these cases could Sudan be found within the eggs, 

 although some of the follicular membranes seemed very faintly 

 stamed. 



This different result during the two seasons is of consider- 

 able mterest from the standpoint of determining the season in 

 which the eggs of turtles grow. If the eggs had been growing 

 (depositing yolk) in January, they should have taken up the stain. 

 The fact that they failed to do so is evidence that they are not 

 growing in January. The definiteness of this finding is somewhat 

 vitiated however, by the writer's observation (09) that the di- 

 gestive capacity of these forms is very low in midwinter; and by 

 the further observation that the forms which were fed the Sudan 

 m winter sometmies regurgitated parts of it. It cannot be stated 

 as certain, therefore, that as much Sudan was put into the blood 

 of the turtles in winter as in summer. (The turtles were kept 

 from summer until January in aquaria containing water at outside 

 temperature. At the beginning of the feeding experiment they 

 were brought into w^ater at summer temperature, about 20^). 



The ovaries from rabbits injected (with the same solution as 

 for the birds) once or twice daily for a week, were examined. Only 

 two of these animals survived the injections longenough to be con- 

 sidered seriously. One of them showed no certain traces of the 

 stain anywhere in the ovaries, the other, only in the corpora lutea. 

 This work on the rabbits was shared by Prof. S. A. Matthews. 



Deposition of Sudan III in the Soma 



Fowls heavily fed on Sudan, for even a day or two, usually show 

 upon exammation a reddish color in all their adipose tissues, most 

 prominentlyin subcutaneous and peritoneal fat. This but confirms 

 Daddi. In addition to his findings, however, it was determined 

 that if newly hatched chicks be fed the stain during the growth of 

 the juvenile plumage the feathers also take up the stain and become 

 distinctly red in color; (the Sudan-containing ofFai was often and 

 completely removed from the brooders and pens to prevent its be- 



