204 W. A. Matheny 



EXPERIMENTS WITH ALCOHOL 



The first series of experiments was started January 6 and carried 

 on until June 4. The percentage of alcohol given, varied from i 

 per cent to yio per cent. 



The diflPerent cultures, with one exception, showed remarkable 

 uniformity in division rate. The exception was the culture in 

 \-^ per cent infusion. From the very beginning and uniformly 

 throughout the experiment these individuals showed a slower rate 

 of division. But since there were three cultures in weaker alcohol 

 solutions and all of them equaled the control in division rate, we 

 conclude that this exception was caused by sources other than the 

 alcohol. 



When the experiment was discontinued the control had reached 

 243 generations, the i per cent culture 245, the to per cent cul- 

 ture 211, and the 2V per cent culture 248. The 5V per cent 

 culture was discontinued in the 227th generation at which time the 

 control was in the 224th. The i-ioo per cent culture was discon- 

 tinued in the 184th generation while the control was in the 178th. 



The second series of experiments jwas started January 26. The 

 percentage of alcohol varied from 2 per cent to 6 per cent. At the 

 end of the 15th day, the 6 per cent culture was dead. During this 

 time it had reached only the 8th generation, while the controlwas 

 in the 22nd. The 5 per cent culture lived 25 days and reached the 

 25th generation while the control was in the 39th. The 4 per cent 

 culture died during the 50th day in the 57th generation at which 

 time the control was in the 82nd generation. , 



When the 6 per cent culture died it was 14 generations behind 

 the control, the 5 per cent culture was also 14, and the 4 per cent 

 culture was 25. 



The experiments were discontinued on June 4. The 3 per cent 

 culture had reached 196 generations and was 24 generations behind 

 the control. It might be noted here that this culture seems to 

 mark the beginning of the effects of alcohol. The 2 per cent cul- 

 ture was in the 22ist generation, and showed no effects whatever 

 from the stimulus. The control was in the 220th generation. 



Depression periods were never in evidence. When the tempera- 



