;26 



A.H. Estahrook 



TABLE XXXVII 



Measurements of groxvth on 1-2,^00 alcohol. 



difference becomes insignificant. So the result would indicate 

 that the alcohol had not increased the growth above the normal 

 amount. 



Groivth in hay infusion of Paraniecia treated for JO minutes after 

 fission u'lth 7| per cent alcohol. To see if treatment with a strong 

 solution of alcohol for a short period would have any lasting effect 

 upon the animals when transferred to normal conditions, or whether 

 the animals would be inhibited in growth for the actual time 

 in which they were in the alcohol, the following experiment was 

 tried: Paramecia just after fission were put into y^ per cent 

 alcohol and allowed to remain. At the age of 5 minutes, and 30 

 minutes, they were shorter than they were at the time of separa- 

 tion. They were also swollen and vacuolated and motionless. 

 Water evidently had been extracted from the Paramecia by the 

 strong alcohol (see table XXXVIII). 



At the end of 30 minutes the Paramecia in the alcohol were 

 taken out and put into hay infusion. Fifteen minutes after 

 being taken out of the alcohol or at the age of 45 minutes, they 

 had increased from 12 1.8 microns (the size at which they were 

 transferred from the 7^ per cent alcohol to the hay infusion), to 

 147.9 microns, being then sightly larger than at the time of fis- 

 sion. No further growth took place in the Paramecia that had 

 been treated for 30 minutes with the alcohol until shortly after 5 

 hours. At that time the Parmecia began to increase in size, and 



