THE PHYSIOLOGt" OF CELL DIVISION 



41 



TABLE 6 



In these experiments both acetic and butyric acids were used. The eggs were exposed, 

 about one hour after removal, to sea-water containing 6 cc. n/ 10 fatty acid to 100 cc. 

 sea-water, for one minute. After ten minutes they were brought into the solutions 

 of the series where they remained thirty minutes; they were then returned to sea- 

 water. Hypertonic sea-water and cyanide were also used for comparison. The 

 figures give the approximate proportion of mature eggs forming free-sivimming 

 blasiulae and gastrulae. 



A. Acetic acid series 



AFTER-TREATMENT 



July 23 



July 24 



July 25 



July 26 



1. None (acetic acid alone) 



2. Hypertonic sea-water 



3. n/lOOO KCN 



4. 5 V. % ethyl alcohol 



5. 2 V. % n-propyl alcohol. , 



6. 0.8 V. % n-butyl alcohol. 



7. 0.25 V. % n-amyl alcohol 



ca. 5 % 

 no exp't. 

 30-40 % 

 30-40 % 

 ca. 20-25% 

 ca. 35-40% 

 ca. 25-30% 



10-15 % 

 ca. 10-15% 

 ca. 90 % 

 ca. 60 % 

 ca. 60 % 

 ca. 70 % 

 ca. 65-75% 



10-15 % 

 ca. 50 % 

 ca. 70 % 

 ca. 10 % 

 25-35 % 

 ca. 50 % 

 ca. 40 % 



B. Butyric acid series 



1. None (butyric acid alone) . 



2. Hypertonic sea-water 



3. m/lOOO KCN 



4. 5 V. ethyl alcohol 



5. 2 V. % n-propyl alcohol... 



6. 0.8 V. % n-butyl alcohol... 



7. 0.25 V. % n-amyl alcohol. . 



10-15 % 

 no exp't 



ca. 20 % 

 85-90 % 



80-90 % 



ca. 60 % 

 ca. 40 % 



ca. 10 % 

 ca. 5 % (de- 

 crease) 

 ca. 50 % 

 ca. 35-40% 



increase 

 slight 



«10%) 

 ca. 10 % 

 ca. 20 % 



20-25% 

 30-40% 



ca. 50 % 

 5-10 % (de- 

 crease) 

 40-50 % 



ca. 50 % 

 ca. 60 % 



ca. 30 % 

 ca. 50 % 



ca. 90 % 

 decrease 



(ca. 10 %) 

 decrease 



(10-15 %) 



ca. 50 % 

 ca. 60 % 



Controls. In all four series the great majority of sperm-fertilized eggs yielded 

 normal larvae. The unfertilized eggs showed no development. 



In three additional similar series with acetic acid, methyl alco- 

 hol (10 V. per cent and 8 v. per cent) and capryl alcohol, CsHiyOH, 

 (one-fifth saturated solution in sea-water) were used in addition 

 to the above. Methyl alcohol proved unfavorable; capryl al- 

 cohol gave a well-marked increase in two of the experiments; 

 in the third the acid treatment alone produced an unusually high 

 proportion of larvae (70 to 80 per cept of the mature eggs) and 

 all forms of after-treatment were unfavorable (cf. table 7). * 



