492 



G. N. CALKINS AND L. H. GREGORY 



D. Conjugations in the T' {Jt.-line) series 



The T' series was started with an ex-conjugant from a wild 

 pair isolated December 13, 1912. The first two divisions took 

 place on the 16th and the 17th, and the four lines were success- 

 fully reared in permanent cultures. The first rich cultures were 

 made on December 26 and the first conjugation tests on December 



Diagram 5 



