Hatai, Spinal Ganglion Cells. 33 



with iron-haematoxylin a deep black to a grey. The iron test 

 shows in the same granules also varying amounts of the iron. 

 These granules are hung along the linin net as is shown in all 

 the figures. By using Biondi-Ehrlich's tricolor stain, these 

 granules stain from a deep blue to a brownish red ; thus all the 

 staining methods employed show that these granules are chem- 

 ically heterogeneous. This gradation in the staining capacity 

 probably indicates gradations in the chemical constitution of the 

 granules which range from a substance rich in nucleic acid to a 

 substance poor in nucleic acid. These granules are found most 

 abundantly along the nuclear membrane, especially at the two 

 poles of the nucleus on the side toward the cytoplasmic process 

 (Figs. I, 4, 5). It is a striking fact connected with the distri- 

 bution of the granules that the appearance of the Nissl gran- 

 ules in the cytoplasm is clearly correlated with the accumulation 

 of the granules at the two poles of the nucleus. The Nissl 

 granules first appear in the neighboring cytoplasm. This fact 

 suggests that the Nissl granules are derived from the nucleus.' 

 From facts similar to those just given, as well as from other 

 evidence, Scott ('98-'99) arrived at the conclusion that the 

 Nissl granules are of chromatic origin and are produced by the 

 migration of the basophile granules from the nucleus. The 

 nuclear origin of the nuclein compounds which are seen in the 

 cytoplasm has been maintained by several investigators from 

 observations on glandular tissues as well as on the muscles. 

 My own observations therefore indicate the nuclear origin of 

 the Nissl granules and thus corroborate the observations of 

 these previous investigators, Holmgren ('99) on the nerve cells 

 of Lophius piscatorioiis and Rohde ('03) on the nerve cells of 

 various vertebrates. 



^ The formation of the NissL granules in the nerve cells is comparable 

 with the formation of the zymogen granules, muscle fibrils, and yolk granules. 

 This is a highly important and fundamental problem in cellular biology and the 

 subject is fully discussed and presented historically in Wilson's "Cell in De- 

 velopment and Inheritance," 2nd edition, 1900. 



