Hatai, Spinal Ganglion Cells. 41 



phile covering^ of the nucleolus, no more chromatic substance 

 can be demonstrated by using ordinary stains like toluidin blue 

 and erythrosin. It is a well-known observation, not only in 

 the early embryonic nerve cells but also in the cells of the active 

 glandular tissues, that preceding the migration of the chro- 

 matic substance from the nucleus to the cell body the substance 

 appears at first along the inner surface of the nuclear membrane 

 whence it passes by either diffusion or migration into the cyto- 

 plasm. But as described above, the nucleus of the adult nerve 

 cell does not show basophile or chromatic substance except as 

 in a thin layer about the nucleolus. 



Very recenly, by applying Zimmermann's "Jodgriinfuchsin 

 method" to the nerve cells in various animals, Rohde ('03) 

 arrived at the conclusion that "Das Enchylema ist farbbar und 

 zwar durch Jodgriinfuchsin wei die Nukleinkorper. Es en- 

 thalt demnach ebenfalls Nuclein, entweder gelost oder in dif- 

 fuser Form." This conclusion is correct also in the case of the 

 white rat. I applied the iron and phosphorus reaction to the 

 adult nerve cells and obtained always positive results from the 

 enchylema which, however, does not stain by using ordinary 

 histological technique. Figure 7 is a drawing of the ventral 

 horn cells in an adult white rat treated by the method for de- 

 tecting iron and phosphorus. As the figure shows, the Nissl 

 granules are directly continuous with the dissolved nuclein in 

 the nucleus. At the two poles of the oval shaped nucleus, the 

 dissolved nuclein is most abundant (Fig. 7) and from these 

 poles it diffuses out of the nucleus into the cytoplasm. By 

 using the technique for phosphorus and iron one is surprised to 

 see the large amount of the nuclein which exists in a soluble 

 condition. Thus one can easily imagine a cyclical interchange 

 of the substances between the cell body and nucleus, since the 

 material to be exchanged is in solution in the enchylema. The 

 application of technique for the detection of phosphorus and 

 iron to the spinal ganglion cells in the adult rat shows the same 

 results and therefore does not need to be especially described. 

 These appearances suggest that in the adult and later stages the 

 Nissl granules are formed in the cytoplasm from the nuclein 



