452 The Vessels of the Limg of Nectiirns Macnlatns 



pushed in and the injecting mass can be seen running rapidly through th& 

 lymph vessels. The vessels are of considerable size, the mass flows easily, 

 and, as there are no valves, but little pressure is necessary. The injection 

 should always be made toward the head. This procedure should, with a little 

 practice, give well filled lymph vessels in the lungs and also in the stomach. 

 "Warm masses do not give as good results as cold. I have frequently filled 

 the lymph vessels of the lungs and stomach with a celloidin mass and have 

 obtained very instructive preparations by digesting in pepsin. 



In my hands granular injecting masses have given the best results, and 

 thus one may avoid the uncertainty of, e. g., such fluids as aqueous solutions 

 of Berlin or Prussian-blue. The lungs and stomach can be dissected out, har- 

 dened in alcohol, cleared in oil of clove, washed out with xylol, and mounted 

 in balsam. By cutting the lungs and stomach open before mounting, the 

 study of the preparation is made easier. 



LITERATURE. 



Hoffman, J. — Die Lungen Lymphgefasse der Rana temporaria. Dorpat., 

 1875. 



Miller, W. S. — Contributions from the Anatomical Laboratory of the Uni- 

 versity of Wisconsin. Bulletin of the -University of Wisconsin, No. 

 33, 1900. 



SucHAED, E. — Des vaisseaux sanguins et lymphatiques du poumon du Triton 

 Crete. Arch. d'Anat. Micros. T. III. 



Structure du poumon du Triton et de la Salamandre maculee. Arch. 



d'Anat. Micros. T. VI. 



EXPLANATION OP PLATES. 

 Plate I. 



Fig. 1. Dorso-lateral view of the stomach and right lung of Necturus. 

 The heavily shaded vessel is the pulmonary artery; the lighter shaded vessels 

 are the lymph vessels. The stomach was empty and contracted when the 

 injection was made. Note the large sinus-like lymph trunk on the dorsal 

 side of the stomach. 



Fig. 2. — Ventral view of stomach and lungs of Necturus. Same prepara- 

 tion as Fig. 1. The heavily shaded vessels are the pulmonary veins; the 

 lighter shaded vessels the lymph vessels. Note the sinus in the diverging 

 angle of the lungs and the large lymph trunk on the mid-ventral surface of 

 the stomach. 



Fig. 3. Lymph vessels of the lung and stomach of Necturus and their con- 

 nection with the veins. The shaded vessels are the lymph vessels; the 

 veins are shown white. The plate is best seen from the side. The connect- 

 ing vessel is indicated by the *. The left lung is reflected to the right and 

 the stomach is partly dissected free. 



Plate II. 



Lung of Necturus with the blood and lymph vessels injected. The lung 

 was cut open, spread out flat, and mounted in balsam. A, artery; V, vein. 

 Only the main branches of the artery and vein are shown. In two places 

 the capillary network is indicated diagrammatically. The blood and lymph 

 vessels were drawn by means of the camera lucida and show the exact rela- 

 tion of both sets of vessels. 



