520 Proceedings of the Association of American Anatomists 



NOTE ON THE STEUCTUKE OF THE MOTOR NERVE ENDINGS IN 

 VOLUNTARY MUSCLE. By G. Cakl Huber. 



In three articles recently published by Sihler, dealing with the ter- 

 mination of nerves in muscle tissues, he has criticised certain observa- 

 tions made by Huber and DeWitt, the discussion having reference to 

 the relation of the sarcolemma to the motor endings. In our work we 

 stated that the endings were hypolamellar. Sihler contends that they 

 are external to the sarcolemma, he basing his statements on observa- 

 tions made on tissues stained by his acetic acid-hgematoxylin method, 

 while our conclusions were based on the study of tissues stained intra 

 vitam with methylene blue, fixed, sectioned, and counterstained in alum 

 carmine. In striated muscle, fixed and hardened in bichloride and 

 stained after Mallory's aniline blue-fuchsin connective tissue stain, the 

 connective tissue and sarcolemma stain blue, while the muscle stains 

 red or orange red. It seemed therefore probable that this method 

 might be used to advantage in ascertaining the relation of the sarco- 

 lemma to the motor ending. One of the small intrinsic plantar muscles 

 of a rabbit's foot was fixed in bichloride solution, cross sections of 3 // or 

 less in thickness were made and stained after the above mentioned 

 method. When such sections were studied under the 1/12 in. oil im- 

 mersion with the Ko. 12 compensation eye-piece (Zeiss), numerous 

 motor nerve endings were observed, in which there seemed no question 

 but that the motor nerve ending in voluntary muscle is under the sar- 

 colemma. 



NEURO-MUSCULAR SPINDLES IN THE INTERCOSTAL MUSCLES OF 

 THE CAT. By G. Cabl Huber. 



That neuro-muscular spmdles are found in the intercostal muscles is 

 ' well known. The present paper deals with the number and distribution 

 of the sensory nerve end organs in the intercostal muscles of the cat. 

 The successive intercostal muscles of one side were removed and stained 

 after Sihler's acetic acid-haematoxylin method. The tissue thus stained 

 was separated into small pieces, each of which was crushed between two 

 slides and the number of the sensory nerve end organs in each piece 

 noted. In the six upper intercostals, from 60 to 100 sensory nerve end- 

 ings were found in each intercostal space, from the 7th to the 10th 

 they were somewhat less numerous, and in the 11th and 12th, 28 and 18 

 sensory nerve endings were found, respectively. Serial sections of the 

 muscular tissue in the 4th intercostal space were made; no special 

 grouping of the sensory nerve endings was observed, and it was found 



