(314 L. E. Cary, 



nearest body of water in which Goniohasis was known to occur. 

 Two of the number were killed on the daj' when the others were 

 lirst fed. and their intestiues examined for the presence of worms. 

 Xo Trematodes were found in the digestive tract, nor in any of 

 the other organs of the body. The three remaining frogs were fed 

 with the intestines of the infected tadpoles from time to time, just 

 as the latter happened to die. One of the frogs was killed a week 

 after the first feeding. In its cloaca were found seven worms. 

 A second frog which had been fed with the infected intestines 

 three times, was killed three weeks after the last feeding. Five 

 worms were found in the cloaca and in the lower part of the 

 intestine of this specimen. The third frog was lost through its 

 jumping out of the jar and drying up in the bottom of the can in 

 which the jar was kept to exclude thé light. 



The number of worms found in the digestive tract of either of 

 the frogs fed with the tadpole intestines was considerably smaller 

 than the whole number of worms contained in the latter at the 

 time of feeding. In neither case was the number in the intestines 

 known with certainty, but in the intestines fed to the first frog 

 twelve worms were counted. As the tadpole intestines were full of 

 dark colored sediment and plant tissues it is probable that some of 

 the worms were overlooked in making the count; as proved to be 

 the case in every instance when the worms were counted in the 

 intestine, and the latter then torn with needles to allow the worms 

 to escape. 



In discussing the transfer of the encysted cercariae of Amphisto- 

 muut suhclavatum to the vertebrate host, Looss says (1892, p. 166): 



"Solche Cysten sammeln sich während des ganzen Sommers im 

 Bodensatze der Gewässer an und werden von ihren Wirthen haupt- 

 sächlich während der Winterszeit aufgenommen. Diese verbringen, 

 wie bekannt, die Frösche im Schlamme vergraben; sie genießen 

 Schlamm und nehmen dabei regelmäßig auch die Wurmcysten mit 

 auf. Bei allen den Fröschen, die ich im Winter und im allerersten 

 Frühjahre erhielt, fand ich oft recht ansehnliche Mengen von 

 Schlamm im Magen und Darm und in diesem bewegten sich stets 

 einige, oft aber 20 — 30 ganz junge Amphistomen von den Größen- 

 verhältnissen der Cercarien : neben ihnen waren zum Teil Reste der 

 (Jysten zu sehen. Auf Grund dieser unzweideutigen Tatsachen hin 

 habe ich es unterlassen, besondere Fütterungsversuche anzustellen. 



