TABLE 1 

 Young egg 



1 The color of the globules is given if they take a different stain from the ground-substance of the 

 sphere. In that case the ground-substance always takes the 'plasma' stain. 



2 Meves' killing fluid for mitochondria is a modification of Flemming's fluid, i.e., the acetic acid con- 

 tent is much reduced (Erg. d. Anat. u. Entw., Bd. 12; Lee, last ed.). 



' Benda's stain for mitochondria is a double stain of sulphaUzarinate of soda and crystal- violet. (Erg. 

 d. Anat. u. Entw., Bd. 12, or Lee, last ed.) 



* The color of the oil depends on the degree of extraction. With long extraction the violet is removed , 

 leaving the blackening caused by the osmic acid in the fixative. 



' Benaley's mitochondrial methods (modification of Altman's methods) : 



a. Killing fluid: 



Osmic acid 4% 2 cc. 



Potassium bichromate 2.5% 8 cc. 1-24 hours 



Acetic acid 2 drops J 



b. Stains: 



Altman's anilin fuchsin 6 min. 



1% methyl green 00 dip 



Wash in 95% alcohol 



c. Bleach to be used after the above killing fluid: 



30 sec 1% potassium permanganate 



30 sec 5% oxaUc acid 



Wash in water 



Dip in 2.5 % potassium bichromate 



194 



