PLASMA-STEUCTURE IN EGG OF HYDRACTINIA 



195 



TABLE 1— Continued 

 Old egg 



6 Five per cent formalin was made in normal salt solution and the formic acid neutralized with so- 

 dium carbonate (Mann,'02). 



' Hot water proves an excellent fixation both for nuclear and protoplasmic structures. 



' The pseudochromatin-granules lie in a protoplasmic net taking the 'plasma' stain. 



' The mitochondria are absent, probably dissolved by the acetic acid of the fixative. 



1" The mitochondria cannot be identified because of the poor fixation given by alcohol. 



n The mitochondria cannot be differentiated because they stain exactly like the other protoplasmic 

 elements. 



'2 Auerbach's stain, used as a chemical test, is a mixture of acidulated methyl-green and acid fuchsin. 

 Jenaische Zeitschrift, Bd. 30, 1890. 



