PLASMA-STRUCTUEE IN EGG OF HYDRACTINIA 207 



seems no more probable that an emission should occur with the 

 chromatin in a diffuse state than when it is in the condensed condi- 

 tion of the early growth-period, at which time no emission could 

 be proved. 



4. It is true that globular masses appear against the nuclear 

 wall which after certain stains may be colored like both the pseudo- 

 chromatin-granules and the chromatin. That they are not the 

 same was shown by Benda's stain and double staining with saf- 

 franin and methyl- violet (table 1). This similarity of staining 

 reactions in some cases makes confusion of the three elements 

 easy. The globules also, as stated above, do not form disper- 

 sion centers. 



5. Although in the majority of cases the pseudochromatin- 

 granules take the basic stains, there is still strong evidence from 

 staining reactions against their chromatin character. Paradoxical 

 as it may seem, the most convicing evidence against their being 

 extruded chromatin is, in fact, from staining tests. 



As can be seen from table 1, which presents the results of 

 staining tests on sections after many fixations, the granules 

 usually stain with the basic stains like the chromatin. For 

 example, the result is the same when iron-hematoxylin is used 

 after any killing fluid, whether it contains osmic acid, a heavy 

 metal, or is an indifferent fluid such as neutral formalin, alco- 

 hol, or hot water. In fact, in these indifferent fluids, the 

 granules stain as intensely as in material fixed in acid fluids, 

 such as sublimate-acetic and picro-acetic fixatives, while they 

 stain much more strongly than in eggs killed in the usual osmic 

 acid containing fixatives. Again, in material fixed in Meves' 

 fluid and stained according to Benda's method, the granules 

 and the chromatin stain alike (i.e., yellow). Also, staining 

 with thionin or Auerbach's fluid gives the same results, the 

 granules take the basic stain. This is the result with the large 

 majority of stains. 



There are, however, some exceptions to this condition. The 

 first fact to come to my notice was the variable results obtained 

 with saffranin and light green when used after Meves' and Flem- 

 ming's killing fluids. In the early stages the nucleus stains with 



