STRUCTURE AND DIVISION OF TRICHOMONAS 139 



blepharoplast. In nearly all cases the new blepharoplast is 

 smaller than the one attached to the old chromatic margin, and 

 it would not be unexpected if it should begin as a small bud 

 from the main or mother blepharoplast. The daughter blepharo- 

 plast continues to separate from the old, until the two are on 

 opposite sides of the nucleus. The paradesmose connecting them 

 remains on the outside of the nuclear membrane which appears 

 to persist during division (fig. 23). Figure 22 shows the two 

 blepharoplasts in place and the spindle forming in the nucleus, 

 while the chromosomes are not quite completely aligned in the 

 equatorial plate. 



5. Other structures. On account of the poor stainability of the 

 anterior flagella and on account of their frequent position on 

 or close to the cell to which they belong, and on account of the 

 presence ofttimes of large numbers of slender bacilli and wavy 

 spirochaetes, the behavior of these structures in division has 

 been difficult to follow. I am convinced, however, that the 

 accounts of other authors are correct to the effect that one or 

 two of these flagella accompany the new blepharoplast, while 

 the other two or one remain with the old or parent blepharoplast 

 (figs. 25 and 26). New flagella to make the full number appear 

 to be formed as new outgrowths from the blepharoplasts (fig. 32). 



Late in the prophase the axostyle becomes separated from the 

 blepharoplast and begins to degenerate (figs. 20 to 22). New 

 axostyles grow out from the blepharoplasts in the telophase, 

 as will be described later. • 



I have not been able to detect any peculiarities in the behavior 

 of the parabasal bodj^ during the prophases. I have drawn 

 figure 16 to show that there cannot possibly be any confusion 

 between the parabasal and the outgrowing new chromatic basal 

 rod. The parabasal is unusually long in this specimen and there 

 is a thin region over the nucleus which suggests that the distal 

 end may possibly become detached. This idea is also suggested 

 by figures 4 and 5, where there is a constriction; but in these 

 latter cases there is no evidence of approaching division. 



I have already suggested that new long rows of chromatic 

 granules grow out from the chromatic basal rod. On the other 



