584 GEORGE H. BISHOP 



from nucleus to cytoplasm becomes least abrupt. The margins 

 of the central vacuoles take the stain more sharply than the ad- 

 jacent cytoplasm — a conditon not obtaining for the periphral 

 vacuoles — and they lie so close to one another as virtually to 

 form a reenforcement to the diffuse nuclear wall, which appears 

 to follow their contour and fill their interstices. The heavier 

 stain in their margins may be due to the presence of the sub- 

 stance of the nuclear vesicle in their surface films. They appear 

 to compress the nucleus, which elongates to two or three trans- 

 verse diameters. The cytoplasm of the central portion, sur- 

 rounding these central vacuoles, takes the stain more heavily 

 than that surrounding what peripheral vacuoles still persist. ^ 



The cell is now in the condition represented by figure D. 

 Material at this stage shows the basophile granules not only just 

 outside the nucleus, but precisely in the areas at the ends of the 

 nucleus from which the nuclear wall has disappeared (pi. 1, figs. 

 8, 9). The diffuse structure and pronounced staining reaction 

 of the region renders difficult the exact location of these granules 

 with respect to the blurred residuum of the nuclear wall. The 

 appearance occasionally is that of a gap pushed outward through 

 the wall between nucleus and cytoplasm, flanked on either side 

 by the fat-globules; the granules contained in the nucleus are 

 escaping through the opening. 



The granules in the cytoplasm increase in number and become 

 evenly dispersed from nucleus to cell-wall. They are not con- 

 fined to the regions of the cells near the ends of the nucleus where 

 they first appear. They seem also to have passed out laterally 

 between the vacuoles in considerable number. These granules 

 enlarge to spherical globules, and at the first the largest lie well 



2 What the difference is between the central and peripheral cytoplasm is not 

 clear, but the different aspect seems to be due to the different distribution of 

 globular or finely emulsified fat. If it were demonstrated that the fat were synthe- 

 sized in this region next the cell wall, and deposited in the cytoplasm in the form 

 of an emulsion, from which it condensed as the fat-content increased to droplets 

 which grew by accretion into vacuoles, then the lighter staining reaction might 

 be assigned to the fine dispersal of fat in the cytoplasm peripherally. This may 

 be a partial explanation, but, as will appear from later consideration of nuclear 

 activity, this central cytoplasm seems to be influenced also by the nucleus more 

 pronouncedly than the peripheral. 



