HISTOLYSIS OF FAT-BODY OF APIS 585 



toward the periphery of the cell, where one might expect to find 

 those which had earliest left the nuclear region. The central 

 fat-vacuoles also recede from the nucleus, and become dispersed 

 among the enlarging granules. The nuclear membrane gradually 

 reappears, as the vacuoles leave the region of the nucleus; but 

 the latter does not reassume its former oval shape; it becomes 

 even more attenuated, sometimes so extended that if straightened, 

 it might touch the opposite sides of the cell. The ends of the 

 nucleus are the last regions to be enclosed. Often these may be 

 seen open in a nucleus of a much later stage of the cell, with small 

 dark-staining granules near the aperture (text fig. E). Cells 

 may be observed in which, instead of two ends, three or more 

 areas of a nucleus appear to have opened out; and a plane section 

 would fail to reveal the extent of this radiate condition of the 

 nucleus in a large proportion of the times it might exist. In the 

 queen pupa, a multipolar extravasation of the nuclear granules 

 is the rule. 



Stage E. The result of these changes is seen in figure 5. After 

 the previous stage the fat cell does not increase in size. Once 

 the nuclear wall is reformed there is no further visible evidence 

 of activity within the nucleus. The wall stains much more 

 sharply than before its dissolution. The large basophile granules 

 are still present in considerable numbers, but the extreme dis- 

 tortion of the nucleus makes difficult a comparison with previous 

 stages as to its size or content. The finer basophile granules are 

 more numerous, relatively to the number of the larger ones which 

 remain in the nucleus, than before the transformation. 



Outside the nucleus, however, the granules undergo a definite 

 development. They enlarge, staining less deeply with basic 

 dyes as size increases, and finally taking an acid stain, until, 

 with the same staining technique as before, the cytoplasmic dye 

 absorbed is often more prominent than the nuclear. They 

 finally become (typically) vacuolated spheres, the granular 

 peripheral shells of which stain slightly darker than the cytoplasm 

 of cells in previous stages, and the centers of which often appear 

 to be dissolved out in preparation much as the fat-globules are. 

 In the meantime both the cytoplasm and most of the vacuoles 



