HISTOLYSIS OF FAT-BODY OF APIS 587 



peripheral cytoplasmic mass (in the interstices of the fat-vacuoles) 

 precipitates upon fixation to a finely reticulate network, the clear 

 interstices of which are large enough to be distinguished under 

 the high power (pi. 1, fig. 3). The central or perinuclear cyto- 

 plasm fixes to a finer granulation, not so fine as the nucleoplasm, 

 but more homogeneous than the peripheral, and considerably 





Text fig. E Fat-tissue cell, stage E — early pupa — characterized by the reforma- 

 tion of the nuclear membrane, growth of the nuclear granules to albuminoid glob- 

 ules, disappearance of the fat-vacuoles, and resolution of the cytoplasmic 

 matrix. 



Text fig. F Fat-tissue cell, stage F — cells ready to disintegrate, medium- 

 stage pupa; albuminoid globules matured, centers of globules acidophile, peripheral 

 granules in their walls feebly basophile, most of the cell cytoplasm absorbed by 

 the globules. 



more densely staining. Some of the peripheral cytoplasm may 

 be carried centrally as the fat-globules approach the nucleus, 

 especially in cells of the queen larva (pi. 1, figs. 4, 8). With deli- 

 cate staining the membrane separating these two masses, of 

 nucleus and cj'toplasm, has no discernible structure or organiza- 

 tion, but appears to be merely a phase border between two im- 

 miscible fluids. The nucleus maintains the globular shape 



JOURNAL OF MORPHOLOGY, VOL. 36, NO. 4 



