PLATE 2 



EXPLANATION OF FIGURES 



Fat-tissue cells from pupae of the honey-bee. X 330 



Figures 11, 13, and 15, safranin-gentian violet with Gram's solution; figure 

 10, polychrome methylene blue and eosin; figures 12, 14, and 16, iron-alum haema- 

 toxylin and eosin. 



10 Late stage D, worker prepupa, nuclear vesicle reforming. The nuclear 

 wall stains sharply with methylene blue; granules and globules of nuclear origin 

 in the cytoplasm fix eosin after they pass the nuclear border, and methylene blue 

 as long as they are within it. The two cells show the two most typical shapes of 

 cell and nucleus of the worker larvae, i.e., bipolar and tripolar. 



11 Stage E, early queen pupa, multipolar nucleus, with reformed wall, 

 albuminoid globules all in about the same stage of development though of different 

 sizes. 



12 Intermediate stage of albuminoid globule formation, stage E, early worker 

 pupa. Granules of different sizes and different stages of development in the same 

 cell, presumably due to succesive emission from the nucleus. 



13 Same as figure 12, different stain. 



14 Unusually large cell from a worker pupa, stage E to F, undergoing dissolu- 

 tion of the cell wall— at upper left hand — before complete elaboration of its 

 albuminoid reserves. 



15 Another cell from the same slide as figure 14, less than 2 mm. away, on 

 opposite side of the intestine; both are of the same size and the same distance 

 from the surface of the tissue as fixed, and both are in the layer of cells next the 

 intestine. The difference is a metabolic one, of unknown causation. In figure 

 15 may be seen two leucocytes, but no actual leucocytic adherence to or attack on 

 fat-cells could be discerned here. The fat-cells had become loosened from each 

 other and had rounded up, and were nearly surrounded by the body fluids. 



16 One of the smaller cells from a queen pupa, showing conditions more typ- 

 ical of the worker pupa (figs. 12 and 13) in greater degree of diversity of develop- 

 ment of globules, fewer number of them, etc., than the larger cells of the worker 

 pupa tissue itself exhibits (figs. 14 and 15). The probable explanation of this is 

 that the difl'erence in development depends on metabolic rate of nutrition, higher 

 in general in the queen larva, and that the smaller cells from the queen larva 

 or pupa developed more slowly, due to isolation from nutriment, etc., than the 

 best nourished of the worker larva's cells. 



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