58 WARO NAKAHARA 



However, Meves ('97), employing various stains, has shown 

 that the microsomes are chromatin and the macrosomes are to 

 be considered as nucleoh, and, although Korschelt ('97) adhered 

 to his previous view in his later paper, the results of subsequent 

 researches by Flemming ('97) and Henneguy ('04) seem to sup- 

 port the view of Meves. 



Maziarski ('11) in the silk-gland cells of different lepidop- 

 terous larvae, observed that the nucleoli migrate from the 

 nucleus into the cell-body, either as separate bodies or in the 

 form of droplets of 'prosecret,' being dissolved within the 

 nucleus and accumulated in vacuoles. This, although' he does 

 not give any evidence except that this material and the silk- 

 fiber in the lumen of the gland show similar reaction to certain 

 stains, he regards as the source of the silk-secretion. 



I found the following conditions in the silk-glands of larvae of 

 Pieris rapae. In the larva just hatched (figs. 1 and 2), the 

 nuclei of the silk-glands are more or less round, showing almost 

 no sign of branching. Such nuclei contains a number of the 

 nucleoli and chromatin granules. The nucleoli are of varying 

 sizes and some of them show such a shape as to suggest that 

 they are in the process of division. They are scattered in the 

 nucleus or sometimes grouped together into a loose mass. Not 

 rarely one or two of them lie close to the border of the nucleus. 

 The chromatin granules are imbedded in the linin reticulum. 

 The nuclear membrane can easily be demonstrated in most 

 cases. However, at places where the nucleoli are approaching 

 closely to the border of the nucleus, it has been impossible for 

 me to detect a nuclear membrane. 



The cytoplasm is homogenous; but granules of different sizes 

 are to be noted more or less surrounding the nuclei. While 

 most of the granules are somewhat scattered, I find a few almost 

 always occur at the outer border of the nucleus, or contiguous 

 to the nuclear membrane, when this is demonstrable. Now, 

 taking into account the facts of the close approach of the nucleoli 

 to the border of the nucleus and of the partial disappearance 

 of the nuclear membrane, we can conceive that a portion of the 

 nucleoli migrate from the nucleus into the cell-body, forming 



