62 WARO NAKAHARO 



increase of the material with the growth of the nucleus. He, 

 however, did not enter into a detailed discussion of the matter. 



The nucleus contains many nucleoli and chromatin granules. 

 The former are of different sizes, distributed in most cases, fairly 

 evenly throughout the nuclear area and sometimes containing 

 vacuoles. The chromatin granules are very fine, and he im- 

 bedded in the linin reticulum. As has already been said by 

 Marshall and Vorhies, the changes occurring in the nucleus dur- 

 ing the secretion are not great, and the nucleus retains the appear- 

 ance here described for the entire history of the secretory activity 

 of the gland. In the cytoplasmic area, however, some remark- 

 able changes are noted to occur. 



Figure 6 shows a cross section of a resting gland, i.e., the 

 gland which has not yet discharged the secretion material, and 

 figure 8 one which has apparently done some discharging. One 

 will notice that the granules of different sizes fill the entire area 

 of the cell-body in the resting gland, while in the case of the 

 other one, the amount of the granules is much reduced. 



The granules in question are apparently identical with those 

 in the case of Pieris, and we can naturally expect them to be 

 derived from the nucleolar material. Close examination of the 

 nuclei with special reference to the nucleoli indicates that this 

 supposition is not incorrect. 



As has been observed by Marshall and Vorhies, the nucleus 

 become very irregular in shape, especially on the side facing 

 the outer margin of the cell. Not infrequently, the condition 

 as shown in figure 9 is to be observed, in which the nuclear mem- 

 brane apparently has disappeared at some part of the surface 

 of the nucleus, and some of the nucleoli are apparently migrating 

 into the cell-body. 



Marshall and Vorhies described the appearance of small 

 dark colored areas scattered irregularly in the cell-body, after 

 glandular activity of five hours. They say that after twenty- 

 four hours of activity, small and somewhat elongated masses 

 of dark colored cytoplasm, running parallel to the vacuoles 

 appeared, while in some cases the masses were also noticed along 

 the inner border of the nucleus. Other very significant state- 

 ments from their paper are the following : 



