492 D. H. WENRICH 



ing condition of the example in cell E with the presence of this 

 appendage in the earher stages. 



In the cases of chromosomes 5, 6, and 9, there is no method of 

 distinguishing distal from proximal end in the earlier stages and 

 therefore no deductions can be drawn as to which plane of sepa- 

 ration prevails in metaphase. 



For the heteromorphic pairs of chromosomes (nos. 1 and 8) 

 there seems to be little doubt that the first division is a segre- 

 gating one, because of the striking difference in shape of the 

 segregating parts. In this respect they are analogous to the 

 unequal pairs found in Phrynotettix and other forms which 

 divide reductionally in the first division. 



/. Additional observations on chromosome organization. Some 

 additional points of interest on the subject of chromosome or- 

 ganization may be indicated. In the first place outstanding 

 chromomeres are much more abundant than in Chorthippus, and 

 this is particularly true of those occurring at the ends of the 

 chromosomes. These polar granules (chromomeres) have al- 

 ready been noted in chromosomes 5, 6, and 7, but they are also 

 prominent in the earlier stages, at least, in numbers 2, 4, 8, and 

 9. The terminal chromomeres are less prominent but easily 

 distinguishable in number 10, still less so in numbers 11 and 12. 

 In most cases, with the gradual condensation ol the entire chro- 

 mosome, these prominent chromomeres become difficult to de- 

 tect in the later stages (cell D, e. g.). In the case of number 2, 

 however, the polar granules are relatively very large and they 

 continue to be recognizable even in the metaphase (cell E). 



Secondly, the presence of the appended bunches of granules 

 (c) on chromosomes 4 and 8, already noted, may be considered 

 further. As characters for identification of these chromosomes 

 they have been mentioned on a previous page. An examination 

 of the pachytene stages shows these appendant structures to be 

 prominent. Figures 22 and 23 (plate 3) show them (c) attached to 

 their respective spireme segments. Chromosome 4 is further 

 identified in these figures by its greater capacity for holding 

 stain. In both of these figures the appendages appear to be 

 attached to only one of the conjugants of each pair of threads. 



