522 CLARENCE E. McCLUNG 



years I am now in a position to give a much fuller account of 

 ihe chromosomal characters of the genus, although I have not 

 yet seen all the species. Much to my surprise some of the new 

 material shows marked departures from the uniformity preva- 

 lent in all my earlier shdes. As a result, some of my generaU- 

 zations are now^ rendered invalid, but it is hoped that fuller knowl- 

 edge may make possible the formulation of principles having even 

 wider application. 



At present I have preparations from five different species, 

 but in this article I shall discuss fully only the three of which 

 earlier mention was made, for it is only in these that multi- 

 ples have so far appeared. The number of specimens in each 

 case, except brevipennis, is possibly sufficiently large to be 

 representative. 



2. The complex of H. speciosus 



The observations recorded for H. speciosus are essentially 

 correct and need only amplification here. It will be recalled 

 that the accessory chromosome is united with one of the tetrads 

 in the first spermatocyte to produce a hexad element. In the 

 spermatogonium the union with one half of the tetrad also exists 

 and is carried over into one of the second spermatocytes. This 

 association was invariable in all the specimens studied, affecting 

 always the same elements. So far as could be observed the 

 unusual relations of the accessory chromosome did not modify 

 its peculiar character and behavior in other respects. The 

 striking appearance of a chromosome, one part of which is con- 

 densed and safraninophilous while the other portion is granular 

 and tinged with the violet in Flemming's tricolor, is presented 

 to our view in prophase and telophase of the first spermatocyte. 

 As was pointed out at the time, an apparent reduction in chromo- 

 some number occurs without there being any real difference in 

 this respect from other Acrididae. The diploid number of 

 twenty-two becomes the normal twenty-three when it is noted 

 that the accessory chromosome, instead of being free, forms one 

 hmb of a V-shaped element, easily distinguished among the 



