MULTIPLE CHROMOSOMES 525 



throughout essentially the same features as mark its history in 

 speciosus. The only observable difference is in the size of the 

 parts. One indi\ddual, however, exhibits a variation in ap- 

 pearance of the multiple, which makes the conditions in Ana- 

 brus, already described ('05), directly comparable to those of 

 Hesperotettix (fig. 23, pi. 4). Instead of the accessory being 

 attached to the end of a rod-shaped tetrad it is joined to one ex- 

 tension of a ring. This relation appears in the prophase and is 

 carried over directly to the metaphase, so that multiple chromo- 

 somes of very different shape from the ones commonly present 

 are produced. Both forms appear, not only in the same animal, 

 but also in a single cyst. It is probable on this account that the 

 variation does not represent any fundamental difference, but 

 only a divergence in some rate of movement of the parts. 



The spermatogonial complex shows practically the same con- 

 ditions which characterize the corresponding stage in H. speciosus. 

 Minor differences of relative size of the elements are the only 

 ones of note. 



Figure 23, plate 4 exhibits the complex in the first spermato- 

 cyte. It is at once noticeable that the chromosomes are strongly 

 of the Hippiscus type and in polar view appear as rings or V's 

 lying in the equatorial plate. The only unusual feature is the 

 tetrad, elongated in a plane of the spindle axis, with the acces- 

 sory chromosome joined to one end at a more or less acute 

 angle. The behavior of the chromosomes during division pre- 

 sents no unusual features and my general description of these 

 types of chromosomes, given at length in a former paper ('14) 

 may be he'd to apply here. The second spermatocyte mitosis 

 likewise is typical for the euchromosomes, and the multiple 

 chromosome behaves like the same structure in speciosus. 



4- The complex of H. brevipennis 



There is nothing in the specimens of this species, so far ex- 

 amined, to indicate the existence of any of the unusual conditions 

 of chromosome association which occur in speciosus, pratensis 

 and viridis. As may be seen by an examination of plate 3, figure 



JOURNAL OF MORPHOLOGY, VOL. 29, NO. 2 



