56 i 8. ROSS 
No one has succeeded in obtaining preparations of the large 
crustacean nerve cells we are here considering which could be 
interpreted as revealing a typical Golgi apparatus, nor have I 
had any better success. Whether it is absent or whether the 
osmotic conditions are such as to make it difficult to obtain com- 
plete impregnations in these giant elements remains for further 
work to decide. Figure 13 is made from a drawing of a cell in a 
Golgi arsenious acid preparation and represents the only type 
of positive stain that has been obtained in large nerve cells. 
It obviously bears little resemblance to the Golgi apparatus as 
we find it in other nerve cells. These isolated elongated ele- 
ments are of a greater order of magnitude than those obtained 
by Poluszynski in Kopsch preparations and may possibly repre- 
sent greatly swollen mitochondria. 
Trophospongium 
In some cells in ganglia treated by the Kopsch method very 
fine blackened bodies, either short or elongate, are evident. 
Some of them are not larger than mitochondria. Some appear 
as very delicate rods varying from very short to elongate fila- 
ments. These seem to have no relation in position to the clear 
spaces between the Nissl bodies, but are distributed promiscu- 
ously throughout the cytoplasm, and are more numerous in 
some cells than in others, while in yet other cells none are ob- 
served. The more delicate of the filaments are usually found in 
the interior of the cell and the larger toward the periphery. In 
some cases those toward the periphery are demonstrably por- 
tions of the trophospongium, a more or less complex framework 
continuous with sheath cells and penetrating into the cytoplasm. 
It seems evident that the very small blackened filaments in the 
interior of the cell are fragments of the trophospongium and that 
the elements of the trophospongial framework are present 
throughout the mass of the cytoplasm even to the center of the 
cell, and that they attain such delicacy as to tax microscopic 
vision. That these filaments are not of the Golgi reticular 
apparatus is indicated by their extreme delicacy, and especially 
by their connection with the framework invaginated from the 
