1() II. HAYS UlLLAKI) 



appearaiire in transverse section are evidently not present. Nile 

 blue still stains the granules with intensity but no stellate forms 

 are visible. In this process, the fat droplets have been removed 

 by the alcohol and also much of the substance which stains with 

 Cresylviolett has apparently been extracted from the fiber or 

 rendered incolorabl(\ In formalin-bichromate material stained 

 as either Weigert or Altmann preparations, paraffin sections, the 

 granules seldom present the stellate form. The w^ing shaped pro- 

 cesses of Kolliker may be dissolved during the process of paraffin 

 embedding. Holmgren, however, has sometimes demonstrated 

 the wing shaped processes by Benda's mitochondrial method. 



The true granules were described above as having a rod shaped 

 form in the breast muscle of the pigeon. This is not to be taken 

 as invariably true. I have also observed in the pigeon muscle, 

 dumb-bell and diplosomic forms in longitudinally cut fibers. At 

 other times the shape is irregular and the granule is not wholly 

 confined to the anisotropic segment. The longitudinal rows of 

 granules in the light fibers of the pectoralis major of the pigeon 

 are placed at greater intervals than in dark fibers though the gran- 

 ules are somewhat smaller in the former. 



The true granules of the pectoral muscles of the bat are similar 

 in number, size, and j)osition to those of the pigeon. However, 

 in the skeletal muscle of most mainmals (dog, cat, rabbit) the gran- 

 ules are of smaller size and fewer in number. In logitudinal sec- 

 tions they may be dumb-bell shaped, rodules, or slender thread 

 structures, either confined to segment Q or extending through the 

 entire distance between adjacent membranes of Krause, Z. They 

 may occur as spherical bodies having a diameter of 1 m or less and 

 situated in segment ./ on either side of Krause 's membrane. 

 The appearance is then similar to that shown for fat droplets in 

 figure 3. The occurrence of ti-u(> granules in segment ,/ is de- 

 scribed by Holmgren ('10) as typical for manuualian skeletal 

 muscle. The st(^llate or irr(>gular forms in transverse sections 

 shown by staining with ( 'resylviolett were of constant occurrence 

 in all the animals used in this investigation. They may be 

 demonstrated in both light and dark fibers, even when granules 

 appear to b(> abs(>nt by the Weigert and Altmann methods. 



