ON THE INTERSTITIAL GRANULES AND FAT DROP- 

 LETS OF STRIATED MUSCLE 



H. HAYS BULLARD 

 From the Anatomical Laboratory , Tulane University of Louisiana 



SEVEN FIGURES 



CONTENTS 



I. Material and methods 3 



II. Nomenclature 5 



III. Relation of interstitial granules and fat droplets to color and structure 



of muscle 6 



a. Light and dark muscle fibers 



b. Relation of light and dark muscle fibers to white and red muscle 



c. Morphology and position of fat droplets , 



d. Morphology and position of true interstitial granules 



IV. General occurrence of interstitial granules and fat droplets 18 



V. Chemical nature of interstitial granules and fat droplets 21 



a. Chemical nature of true interstitial granules 



1. Refractive character 



2. Solubility 



3. Results with Cresylviolett R R, and Cresylechtviolett 



4. Results with the methods of Weigert, Altmann, Benda and 



Re gaud 



5. Results with acid fuchsin 



6. Results with Sudan iii, osmium tetroxide, and gold chloride 



7. Summary 



b. Chemical nature of fat droplets 



1. Refractive character: Double refraction 



2. Solubility 



3. Results with Scharlach R and Sudan iii 



4. Results with osmium tetroxide 



5. Results with the Nile blue method 



6. Results with the methods of Benda, Fischler, and Klotz for 



free fatty acids and soaps 



7. Results with the Weigert method and related methods 



8. Formalin fixation 



9. Summary 



VI. Physiological significance of interstitial granules and fat droplets.... 39 



VII. Summary and conclusions 42 



Literature cited 44 



1 



THE AMERICAN JOURNAL OP ANATOMY, VOL. 14, NO. 1 

 NOVEMBER, 1912 



