66 R. H. WHITEHEAD 



of material which had been subjected to this treatment, but inas- 

 nmch as Faure-Fremiet, Mayer and Shaeffer* have reported that 

 some combinations of proteid and fat are precipitated by for- 

 maUn, the point was tested by staining paraffin section of formaUn 

 material with Sudan. The results were always negative; even 

 the strong solution of Sudan made by the addition of caustic 

 soda, according to Herxheimer's method with scharlach, left the 

 granules unstained. 



Various attempts were made to verify the hypothesis that the 

 granules consist of a combination of proteid with fatty material 

 by staining methods intended to demonstrate the granules and 

 the lipoid globules in different colors in the same cell, but as none 

 of these yielded decisive results, I may omit a detailed description 

 of them, and state the results of two which were, at least, strongly 

 suggestive. Pieces of formalin material were treated for three 

 days with Flemming's fluid, imbedded in paraffin, sectioned, and 

 the sections stained with neutral gentian and mounted in xylol 

 balsam. In such sections the lipoid globules as well as the gran- 

 ules were stained by the gentian, but the violet color rapidly faded 

 out of the globules leaving the granules stained violet. Again, 

 in sections of material fixed by Ciaccio's method and stained with 

 Sudan and iron haematoxylin lipoid globules were stained red, 

 while here and there among them were small granules stained 

 by the haematoxylin. It did not seem possible, however, to iden- 

 tify these latter absolutely with the granules in question, because 

 it is known that some lipoids after treatment with potassium 

 bichromate give a lake with haematoxylin; and so these haema- 

 toxylin-stained grains may have been simply such lipoids without 

 any proteid admixture. Thus the large amount of lipoids in 

 the cat's testis introduced so much confusion into the picture 

 that it did not seem possible to get decisive results from this 

 method of investigation. Accordingly recourse was had to the 

 pig's testis, as a previous study^ of it had shown almost entire 



' Faur6-Fremiet, Mayer et Shaeffer. Sur la microchemie des corps gras. 

 Arch. d'Anat. Microsc, T. 12, 1910. 



' R. H. Whitehead. Studies of the interstitial cells of the testis. Histology. 

 Anat. Rec. of Amer. Jour. Anat., vol. 7, no. 4, 1908. 



