44 Florence R. Sabiu. 



The first theory, that the lymphatics arise from tissue spaces, 

 received its strongest support from Budge. ^ 



In his first paper Budge described injections of Berlin blue into 

 the false amnion of three-day chick embryos. He found that the 

 injection mass ran out into the area vasculosa in a series of irregular 

 canals forming an abundant network immediately under the epiblast 

 and hence dorsal to the vascular layer. This network of canals 

 extended out to a marginal canal around the area vasculosa similar 

 to the marginal vein. Budge inteq^reted this system of canals as 

 a primitive .lymphatic system which in his injections arose in con- 

 nection with the ccelom and its extra embryonal expansion, the false 

 amnion. This primative lymphatic system he said never had any 

 connection with the veins, so that the interchange of fluid must have 

 been through the walls. Dr. Mall- has studied Budge's specimens 

 and is convinced that they are injections showing simply the extent 

 of the extra embryonal coelom. 



I repeated Budge's experiments, using India ink instead of Prus- 

 sian blue, as it flows more readily, and found that I could duplicate 

 Budge's figures exactly.^ The fluid ran out in blunt processes 

 simulating canals, but readily distingiiished from the lymphatic 

 injections. The fluid runs exactly as it would, if forced between 

 two glass plates held closely together, that is, blunt processes push 

 out which form an advancing network, but this network soon fills 

 into a solid mass. With a careful injection of true lymphatics on 

 the other hand the individual vessels often remain absolutely distinct 

 from the very point of the needle as is shown in Fig. 4, of the article 

 in Volume I, of the American Journal of Anatomy, where the 

 needle was introduced into two places, one just over the shoulder and 

 the other over the crest of the ilium. The injections in the area vas- 

 culosa of the chick are like the pictures obtained by injecting into a 



'Budge. Ueber ein Canalsj'stem im Mesoderm von Hiihnerembryonen. 

 Arch. f. Anat. u. Phys., Anat. Abth., 1S80, s. 320. 



Untersuchungen uber die Entwickeluug des Lymphsystems beim Huhner- 

 euibryo. Arch. f. Anat. u. Phys., Anat. Abth., 1887, s. 59. 



^Buck's Handbook of Medical Sciences. The Ccelom. 



=Sabin. The Development of the Lymphatic System. American Journal 

 of Anatomy, Vol. I, 1901-1902. 



