382 



GRACE MEDES 



Experiment 12. July 9, 1915 



a Sea-water 



b 99.8CC. S. W. + 0.2 cc. 0.45iM NaaCO, 



c 99.6 cc. S. W. + 0.4 cc. 0.45M NaaCOs 



d 99.4 cc. S. W. + 0.6 cc. 0.45M NazCOs 



e 99.2 cc. S. W. + 0.8 cc. 0.45M NaoCOa 



/ 99.0 cc. S. W. + 1.0 cc. 0.45M NajCO, 



g 98.8 cc. S. W. + 1.2 cc. 0.45M NajCOs 



The following data were obtained: 



Time after insemination 



A somewhat similar experiment was performed with NaOH 

 and the effects at a still earlier stage noted. 



Experiment 13. July 29, 1915 



a Sea-water 



b 99.33 cc. S. W. + 0.66 cc. N/10 NaOH 



c .98.66 cc. S. W. + 1.33 cc. N/10 NaOH 



d 98.0 cc. S. W. + 2.00 cc. N/10 NaOH 



The following data were obtained: 



Titne after insemination 



a 

 b 

 c. 

 d 



2.5 MINUTES. 

 PER CENT WITH 

 FERT. MEMBR. 



42.6 



63.7 

 81.5 

 27.1 



1 HOUR, 20 MINUTES. 

 PER CENT IN 2 CELL 



4.1 

 0.8 



98.8 



1 HOUR, 20 MINUTES. 

 PER CENT IN 4-CELL 



95.9 



99.2 



97.4 



1.2 



1 HOUR, 20 MINUTES. 

 PER CENT IN|8-CELL 



2.6 



