STUDIES OF AMITOSIS ' 485 



G counter-stain, or Ehrlich's haematoxylin followed by a strong 

 aqueous solution of eosin. Some few larvae were treated with 

 Flemming's chromo-aceto-osmic fixer and his triple stain. In 

 some cases whole mounts of the tissue were very useful in 

 checking up the observations on sections. In preparing such 

 mounts, the best result was obtained by fixing pieces of the 

 tissue in Carnoy's '6-3-1,' which dissolves the fatty contents 

 of the tissue at the same time, and staining with Grenacher's 

 borax carmine, followed by a differentiation with acid alcohol. 



In order to demonstrate fats in the cell, very small pieces of 

 tissue were thoroughly fixed in osmic add and prepared as paraf- 

 fin sections. Some other materials were fixed in formalin, cut, 

 using the freezing microtome, and stained with Sudan III. 



I used Gage's iodine method (Gage, '06) as the simplest and 

 most convenient for the demonstration of glycogen, and diges- 

 tion experiments by means of saliva were applied as a control. 



I have been able to demonstrate mucus (?) granules in the 

 tissue by the following technique: 



1. Material fixed in picric alcohol for 24 hours. 



2. Paraffin sections fastened to the slides with albumen fixa- 

 tive, without using water to float the sections (a rather large 

 quantity of the fixative was used to insure the sections as free 

 from wrinkles as possible). 



3. The fastened sections treated with xylene and then with 

 95 per cent alcohol as usual, but without being placed in water. 



4. Stained 10 to 20 minutes with alcoholic mucicarmine. 



5. Sections then washed with 95 per cent alcohol and mounted. 



III. THE RELATIONS OF THE AMITOTIC NUCLEAR DIVISION 



.4. Structural changes in the adipose cell 



a. Review of Perezes work. Structural changes in the adipose 

 cells during the larval life of insects have been studied to a cer- 

 tain extent by Wielowiejsky ('83), Van Rees ('88), Karawaiew 

 ('98), Koschevnikov ('00), Terre ('00), Anglas ('00), Berlese 

 ('99, '01), Henneguey ('04), Philiptschenko ('07), Hufnagel Cll), 

 Feytaud ('12) and others, especially in connection with the 



