488 WARO NAKAHARA 



large, measuring about 60 to 70 micra in diameter. Fatty 

 droplets (fig. 9) and glycogen (fig. 10) are present in large quanti- 

 ties. The albimiinous granules are greatly increased in amount 

 (fig. 11), and almost all of the adipose cells are filled with such 

 granules in the larva of complete maturity. 



In full grown larva, many cells contain two or more (rarely 

 up to five) nuclei. Some of the albuminous granules begin to 

 show dark dots, taking basic stain, indicating that the transfor- 

 mation of albuminous substance into urate is beginning to take 

 place (fig. 12). This possibly may be regarded as one of the 

 first signs of histolytic process. Soon afterward, just before 

 the larva enters into the prepupal stage, the nucleus loses its 

 membrane and its structure becomes more or less indistinct 

 (fig. 13). This is, I believe, the sign of a karyolytic process, 

 which concludes the activity of the larval adipose cells. 



B. Some plasma structures 



a. Fat. This is perhaps the most important of all the inclu- 

 sions in adipose cells. As pointed out in the preceding section, 

 the increase in amount of the fat in the cell keeps pace with that 

 of the cell in size. In other words, the amount of fatty inclusion 

 in the cells increases with the growth of the larva. 



We do not know the cytological relations of the accumulation 

 of fat in cells. However, since it is evident that fat is not taken 

 up by cells as such, it is necessarily true in one sense, that cells 

 elaborate the fat within them from its modified forms. In pass- 

 ing, I may mention that very rarely, small fat droplets were 

 seen lying within nuclei. Figure 14 represents such a condition. 



b. Albuminous granules. "Vhe appearance of peculiar spherical 

 granules in the cytoplasmic area of adipose cells toward the 

 close of larval life of insects has been noted since the early year 

 of 1864 (Weismann) by many writers. Going over the descrip- 

 tions by Koschevnikov ('00), Anglas ('00), and others, it seems 

 that some of the spherical granules which have been familiar to 

 the earlier authors, correspond to the albuminous granules, but 

 it was not until the time of Berlese ('99, '01) that granules of 



