490 



WARO NAKAHAEA 



did not note any such substance passing out of the nucleus, 

 but he strongly maintained the endogenic origin of albuminous 

 granules, and suggested that they may be of nuclear origin, be- 

 cause the granules first appear laying very close to the nucleus. 



Examining the cells containing the albuminous granules in 

 the cytoplasmic area, a different kind of granules can in most 

 cases be distinguished from the chromatin and nucleoli within 

 the nuclei (fig. 11). These peculiar granules are similar in ap- 

 pearance to nucleoli, but are distinguished from the latter in 

 showing stronger affinity for certain acid and weaker for basic 

 stains, and hence they may be tentatively called acidophile 

 granules in the nucleus. 



The acidophile granules in the nucleus and aljbuminous gran- 

 ules always show similar staining reactions as can be seen in 

 the following table: 



Delafield's hematoxylin, eosin, and pic- 

 ric acid 



Ehrlich's hematoxylin and pico-fuchsin. 



Iron-hematoxylin 



Flemming's triple 



Ehrlich's hematoxylin, strong aqueous 

 eosin 



Dark 

 olive 



Orange 



Black 



Red 



Pink 



ACIDOPHILE 

 NUCLEAR 

 GRANULES 



Orange- 

 yellow 



Yellow 



Gray 



Orange 



Pink 



ALBUMINOUS 



GRANULES 



Orange 

 yellow 



Yellow 



Gray 



Orange 



Pink 



Still more interesting in these cells is the condition of the 

 nucleus represented in figure 15. Here an acidophile granule is 

 apparently migrating from the nucleus into the cell-body through 

 the spot of the nuclear membrane, where the latter has dis- 

 appeared, thus forming an opening. Since this phenomenon 

 occurs very commonly, it may be regarded at least as one of the 

 possible methods of formation of the albuminous granules. 



As far as the evidence available indicates, the theory of the 

 endogenic origin of the albuminous granules in the adipose cells 



