492 WARO NAKAHARA 



relations of the glycogen in adipose cells. Suffice it to say that 

 the elaboration of glycogen is one of cell activities, and it is not 

 improbable that the nucleus may take some kind of role in this 

 process. 



e. Mucus (?). A peculiar substance, hitherto unknown in 

 the adipose tissue of insects, has been discovered in well-grown 

 larvae of some Lepidoptera. The substance is represented by 

 globules (fig. 16), scattered within the cell-body and respond to 

 some extent to histochemical tests for mucous substance. Picric 

 alcohol preserves the globules, and the latter take mucicarmine. 

 The sections, however, will not show any such globules, should 

 they be placed in water or aqueous solutions, indicating that the 

 substance is very easily dissolved in water. If the substance be 

 really a mucus, it would seem that adipose cells ha,ve the func- 

 tion of secreting this substance. This, together with the ques- 

 tion of exact periods of its appearance in the tissue, however, re- 

 main to be determined by future study. 



C. Nuclear division 



a. Amitosis. The occurrence of bi- and multinucleate cells 

 in larval adipose tissue has been noted for many years, and such 

 authors as Terre ('00) and Anglas ('00) considered them as due 

 to amitotic division of nucleus, without a following division of 

 cell-body, and indicating a senile condition of the cell. Perez 

 ('02) was first inclined to think that the multinucleate condition 

 of the cell is due to amitosis of the nucleus, but later ('10), in 

 his work on Calliphora, he maintained the view that it repre- 

 sents the incomplete separation of the cell-body after the division 

 of the nucleus at the embryonic stage. He did not observe any 

 nuclear figure which could be considered as representing one of 

 the division stages. It is obvious, however, that Perez's later 

 interpretation must be discarded, because, while we can hardly 

 detect a single binucleate cell in the young larva, bi- or multi- 

 nucleate cells are very common in older larvae; whereas we 

 should expect as many multinucleate cells in young larvae as 

 in older ones, if Perez were right in his interpretation. 



Logically, bi- or multinucleate cells may .possibly result in 



