PARASITES OF 
LEPIDOPTERA 
Bombyx mori, all instars............ 
Catopsila ewbule, larva....:.:....<.. 
Danis erippe, larvacwten. 4. 3. ..: 
Danais gelippus, larva 
Dionogaino larva epee eer 
Ephialtes angulosa, adult........... 
Gastrophilus neustria, larva......... 
Lophocampa flavostica, larva 
Mechanitts lysimnia, larva.......... 
SIMULIUM LARVAE 
63 
.Nosema bombycis Nag. 
Glugea eubulis L. and 8. 
.Glugea erippe L. and S. 
Glugea erippe L. and 8. 
Glugea janoris Los. 
.Nosema ephialtis L. and 8. (’08) 
Nosema bombycis Nag. 
Glugea lophocampae L. and §. 
Glugea lysimniae L. and 8. 
Scea auriflamma, adult............. 
COLEOPTERA 
Balaninus amaryllis, larva.......... 
Otiorhynchus fuscipes, larva 
Nosema auriflammae L. and 8. (’08) 
Glugea stempelli Pérez (’05) 
Mycetosporidium talpi Legér and 
Hesse (’05) 
ORTHOPTERA 
Periplaneta americana,............. 
CRITE SHALCHTBNGAES <2 + wlocin 2 5 eee 
NEUROPTERA 
PO MREMEEILG 1QNALG... . 6... 0.2 da ad 
_Glugea periplanetae L. and S. 
_Duboscquia legerit Pérez (’08) 
.Gurleya legeri Hesse (’03) 
MATERIAL AND METHODS OF STUDY 
Simulium larvae from infested streams were frequently col- 
lected and brought to the laboratory, but since parasitised larvae 
could never be kept alive for much longer than over night, the 
majority were killed at once, a few being kept for dissection. 
Killing for sectioning. The best fluid for this purpose proved 
to be one recommended by W. Kahle (’08) which is made up as 
follows: water, 30 parts; 96 per cent alcohol, 15 parts; 40 per cent 
formalin, 6 parts; glacial acétic acid, 1 part. 
A fluid reeommended by Stempell (’09) consisting of corrosive 
sublimate (saturated aqueous solution) 2 parts; 95 per cent 
alcohol, 1 part; and a trace of acetic acid, also gave good results, 
but for subsequent staining with iron haematoxylin was inferior 
to Kahle’s fluid. Either fluid was used by being brought to a 
temperature of about 70° C., when the larva was immersed and 
allowed to remain in the cooling fluid for about fifteen minutes. 
To insure penetration the larval skin was punctured with a fine 
needle as soon after death as possible. 
Killing for smear preparations. Kahle’s fluid poured over the 
smear gave good results, but a method recommended by Perrin 
