70 E. H. STRICKLAND 
it is drawn away from the walls of the spore around the equa- 
torial region as shown in figure 14, etc. With iron haematoxylin 
all of this stains very deeply and uniformly as though it were 
all of a chromatic nature, and it would seem that it must have 
been to some similar appearance in the spore of Glugea varians 
Léger that Vaney and Conte (’01) referred when they likened 
the nucleus of this species to a double T. I was unable to find 
more than two nuclei in these spores. In the spores of Thelo- 
hania giardi Mercier (’08) found five nuclei, and in those of 
Nosema bombycis Stempell (09) found four. In some of the 
specimens there were, however, indications of two darker specks . 
one on each side of the vacuole as shown in figure 22. These 
would correspond to the shell nuclei of Stempell (09) but I was 
unable to distinguish any red coloration. As before stated it is 
not improbable that the spores were immature, and it is quite 
possible that the two nuclei I was able to demonstrate undergo a 
further division before maturation. At any rate, this seemed to 
be the case in the spores of another species studied. At a later 
stage the cytoplasm is once more pressed back againt the walls 
of the spore (pl. 2, figs. 16 and 23). - This is due to the formation 
of the polar capsule within this area. As I never found any 
evidence of this structure projecting into the vacuole I have 
adapted the diagram of a ripe spore of N. bombycis as given by 
Stempell (’09) to the form as shown in pl. 2, figure 23. Full 
details of its growth could not be seen, since it is entirely sur- 
rounded by deeply staining protoplasm. A small depression in 
the protoplasm appears between the two nuclei, at the end of 
the spore opposite to the vacuole, which increases in depth until 
it forms a pear-shaped cavity within the protoplasm. In an 
end to end view of a spore it is seen that the capsule nearly or 
quite reaches the vacuole, for by focussing up and down one can 
see right through the spore (fig. 17). The outline of the capsule 
and its contained filament with the pore in the shell through 
which it is ejected, were not seen, nor in stained specimens was 
the filament ever found attached to a spore as depicted in plate 
2, figures 23 and 24. These and accompanying figures of the 
series are purely diagrammatic. The filament is readily detached 
