74 E. H. STRICKLAND 
gram of the spore can be constructed as shown in figure 26, in 
which the four nuclei, as yet incompletely divided, are indicated, 
while the polar capsule, projecting far into the vacuole, contains 
the greatly elongated coiled filament. This filament, when ejected. 
by the action of iodine, attains the comparatively enormous 
length of thirty to forty times that of the spore (pl. 3, fig. 25). 
It should be borne in mind that, as Stempell points out, this 
filament must, from the manner in which it is ejected, consist of 
a hollow tube which. has to be entirely everted during its emer- 
gence from the spore! When it first appears it may be in the 
form of a loose spiral (fig. 23) but this quickly disappears as the 
filament soon straightens out. This filament is not so readily 
separated from the spore as is that of G. bracteata, but in the 
few cases where it was seen to be detached the basal portion was 
swollen up into a knob (fig. 24) as in that species. In all the 
spores in which the filament was protruded it was noticed that 
the spore contents lost their regularity. J was unable to dif- 
ferentiate the parts, but it seemed that the polar capsule shrank 
and became attached to one side of the spore, while the proto- 
plasm and nuclei settled down into a small area at the narrow 
end of the spore (figs. 24 and 25). 
Macrospores. These were not numerous among the typical 
microspores, and appeared to be somewhat abnormal, for the 
shell was much thinner than that of the microspores (pl. 3, fig. 
22). When treated with iodine none of them was seen to eject 
a filament. 
This species of Glugea seems to be related to G. varians Léger 
(97). The spores, however, are somewhat smaller and the fil- 
ament is proportionally about twice as long. Léger and Hagen- 
muller (’08) state that in G. varians the development of the spores 
is either octosporie or polysporic. This does not appear to be 
the case with G. fibrata, for though [ found on two occasions 
16 sporoblasts adhering together, this did not have the appear- 
ance of being a normal condition. 
The host of G. fibrata is the larva of Simulium bracteatum 
and Simulium hirtipes (?). In infests about 5 per cent of the 
larvae. It was found both in the Arnold Arboretum and in 
Franklin Park, at Forest Hills, Massachusetts. 
