PROCESS OF FERTILIZATION IN THE CRAB 1: 
faintly stained. This treatment makes it possible to find these 
minute structures in the relatively immense egg. After one has 
become familiar with these structures and their position on the 
egg it is possible to find them quite readily with other stains such 
as Delafield’s hematoxylin, iron-hematoxylin, or safranin and 
Lichtgriin. 
The relation of the spermatozoa to eggs taken from the lumen 
of the ovary is shown in figures 88 to 92. Spermatozoa in the 
same condition are also found in eggs taken from the oviduct as 
is shown in figures 93 and 94. Now, when these figures are 
compared with figures 86 and 87, which have been treated with 
the same fixing reagents, it is evident that it is the everted por- 
tion of the spermatozoon which has gone through the shell of 
the egg. The nuclear cup (n.c., figs. 91 and 92) is on the out- 
side of the shell. The everted tubule forms a vesicle within 
which one sees the inverted capsule (znv.c., fig. 91). Hereafter 
I shall call this everted tubule and capsule, the ‘sperm-vesicle.’ 
At the inner-end of this sperm-vesicle the ejected central body 
may be seen (figs. 88, 91 to 94, c.b.). That the part which 
remains outside is the nuclear cup with its radiating pseudopodia 
can be more clearly seen by a surface view of the structure as 
it lies upon the egg, such as is presented in figure 95. Further- 
more, the staining reactions are in accord with those observed 
in the mature spermatozoon and the artificially exploded ones 
and are as follows: 
the part outside of the shell, blue 
the part inside of the shell, red 
part outside of shell, red 
part inside, green mixed with red 
part outside of shell, black 
part inside, brown except the central body which is black 
Thionin and eosin { 
Safranin and Lichtgriin { 
Tron-hematoxylin { 
Koltzoff (06) claimed that in certain Decapods the sperma- 
tozoa settled on the egg with the nuclear cup towards the egg 
and the capsule pointed away from the egg. He was also of 
the opinion that the rebound from the explosion of the capsule 
was sufficient to drive the nucleus into the egg. On eggs taken 
