392 GEORGE T. HARGITT 
where there appears to be little doubt that there is an early sepa- 
ration of the primordial germ cells. Just as the weight of evidence 
in this form is admitted, so it must be in Campanularia. 
3. Later development of the egg 
a. The cytoplasm. Reference has been made to the fact that 
in the entoderm cells which are just starting their development as 
egg cells, there is little or no difference in the appearance of the 
cytoplasm from that of other cells. Figure 2, for example, shows 
at a and b two developing egg cells and one other entoderm cell; 
also some ectoderm cells, in all of which the cytoplasm is the same 
in appearance. But very soon there occurs the change which 
has been familiar to all workers for a long time, namely, that the 
cytoplasm which is granular becomes more compact and the stains 
take hold with greater intensity (figs. 3 to 5). Whether this is 
due to physical or to chemical changes within the cytoplasm is 
not certain; chemical changes are assuredly taking place and the 
physical configuration is also altering. No further changes occur 
while the egg is in its place of origin, but when it is migrating the 
short distance up the pedicel of the gonophore into the latter, it 
is Increasing in size, due to the absorption of food from the enteric 
cavity. In a stage like that shown in figure 6, in which the egg 
is in the base of the gonophore, and figure 7, which represents an 
egg in its final position within the gonophore, the cytoplasm 
shows well marked changes which appear as irregular spaces 
within the cytoplasm. This is not purely an artefact (though it 
may be in part) since the spaces show some definiteness, as though 
caused by currents or streams within the cytoplasm. At the same 
time there is certainly an exchange of substances between the 
nucleus and the cytoplasm, as shown in figures 6 to 8. 
All this time the cytoplasm has remained finely granular, 
with few or no protoplasmic bodies, even at the time when the 
egg has grown to be a quarter of its final size. This growth occurs 
very rapidly up to this point, and I interpret the lack of deuto- 
plasmic bodies as meaning that food taken into the body of the 
cell is used up immediately, with nothing left over for a reserve. 
