394. GEORGE T. HARGITT 
double stains of acid and basic reactions, this body assumes some 
of the protoplasmic tint. In these same figures the nucleoli of 
the entoderm and ectoderm cells are much larger than those in 
the egg cells, though with the nucleus the opposite is true. Until 
the eggs assume their place in the gonophore, the nucleolus 
remains single; sometimes it is present as a single body after the 
eggs have come to rest and have increased in size in their perma- 
nent place in the gonophore (fig. 1). In the short migration 
which the egg cells go through from the pedicel of the gonophore 
into the body of the latter certain changes occur, principally in the 
cytoplasm and nucleus. The chromatin thread of the youngest 
eges disappears and the nucleus contains only a delicate reticulum 
in which, as figures 5 and 6 show, are several deeply staining 
strands. In figure 6 there is also shown a darker and denser 
mass of cytoplasm just outside the nucleus, and scattered through- 
out this, but principally close against the nuclear membrane, 
several very deeply staining small granules. These are of in- 
terest because of what happens in later stages and are explained as 
being due to some substance passing from the nucleus into the 
cytoplasm. 
After the eggs have reached their place in the gonophore, the 
nucleolus soon undergoes great changes, consisting of the breaking 
up of the nucleolus into pieces of various sizes and shapes, and never 
again in the history of the egg is the nucleolar matter in one 
body. It is the history of the changes of these nucleolar bodies 
and their possible significance that this section hopes to describe 
and make clear. Concerning the staining reactions of these frag- 
ments the following will show in general, and may suggest some- 
thing of the nature of the changes involved. Double stains such 
as hematoxylin and eosin, hematoxylin and picric acid, picro- 
carmine and Lyons blue, hemalum and eosin and so forth, show 
some selective action. The result is that some of the nucleolar 
fragments show the colors assumed by the protoplasmic portion 
of the cell, some those assumed by the chromatic constituents of 
the nucleus, and some a tint intermediate between, or rather com- 
pounded from, the two tints. That is, the nucleoli behave as 
chromatic material, as non-chromatic material, or as a mixture 
