490 E. ELEANOR CAROTHERS 
the smaller size of the polar granules, described by Pinney (’08) 
for Phrynotettix. This point is especially in favor of Arphia, 
since part of the chromatin which produces the unequal tetrad 
passes through the greater part of the growth period, and other 
stages of general diffusion, in a dense condition where it appears as 
nothing less than one of the so-called plasmasomes or nucleoli so 
often described for these stages. In this condition it is liable to 
be confused with the ordinary large polar granules of Brachystola. 
My earliest study of the growth period impressed me with the 
strong probability that these bodies which at certain stages stain 
like chromatin, are really composed of chromatin. Further study 
convinced me that at least one of them is associated with the 
unequal tetrad. and this conviction led to the present study. 
Apical cells contain at least one, perhaps more, clear, straw- 
colored, more or less spherical vesicles. Close inspection reveals 
a minute, deeply staining granule at one point of the periphery; 
and further that this point is always in connection with a mass 
of chromatin (fig. 7, k). These latter, the peripheral granule 
and the connection through it with the ordinary chromatin, are 
absolutely characteristic features of such vesicles wherever they 
are found. Since they may or may not be filled with chromatin, 
the staining capacity is a variable factor. . 
At least two of these vesicles are present in the cells surround- 
ing the apical cell (fig. 8, &). In favorable preparations they may 
be traced in the several spermatogonial generations from early 
telophases (fig. 9k) to later prophases (fig, 10k). The one repre- 
sented in figure 10 is connected to a chromatin thread with 
unequal arms, and, as telosynapsis of the elements which form 
the unequal tetrad of the first spermatocytes occurs at a stage 
even preceding this, as is clearly shown’ by figure 11, c, there is 
little doubt that this, also, is the precocious tetrad and its asso- 
ciated vesicle. 
During the growth period there are three vesicles; two single 
and one: double (fig. 13, k, k, k) (occasionally, one-half of the 
latter splits again forming a tripartite vesicle). For this reason, 
and especially since no thorough study of the earlier stages was 
undertaken. the largest number found at that period—two— ° 
