Grafting of Tubularia 



333 



third cut divided the proximal component in half. The piece Ay 

 between the first and second cuts, differed from C not only in 

 position, but in time of exposure of the ends. Both ends of A 

 were exposed at the same time, but the aboral end of C was exposed 

 twenty-four hours before the oral end. In this experiment the 

 graft is in the middle piece (Fig. 9, B). 



Fig. 9 



—2 



Fig. 10 Fig. ii Fig. 12 



— 2 



Fig. n 



w 



-2 — 

 A 



—2 



X 



—2 



Fig. 14 Fig. 15 



Fig. 16 



Taking the piece B first as it is made of the two equal compo- 

 nents grafted together in the same direction (Fig. 9) and com- 

 paring its later behavior with that of A in the first experiment, 

 we find that the effect of the second operation is not so evident. 

 Twenty-four hours after the piece had been separated from A and 

 C, on 75 per cent of the pieces new hydranths were developing on 

 the oral ends of the basal component, and on less than 50 per cent 

 of the grafts oral hydranths were appearing on the distal compo- 

 ponents. The two components rarely acted as one piece, for one 

 or more hydranths usually developed in the region of the graft. 

 In the following table the results from twenty grafts are given. 

 That part of the graft which came from the distal component is 

 designated as D, that from the proximal component as P (Fig. 9). 



If we compare Table 5 with Table i, it will be seen at once that 

 there is no marked difference in the number of hydranths formed 



