398 



Ralph S. LilUe 



TABLE IV— Continued 



No. 



Time after 



removal 



minutes 



{ca.) 



Series I 

 July 31, 1907 



Series II 

 August 7, 1907 



Series III 

 August 12, 1907 



25 



30 



35 



40 



45 



50 



{Vesicle almost disappeared (As in Experiment 4; only {Germinal vesicle disap- 



in nearly all) More fav- one-third or so maturing)] pearing in ca. one-half of 



orablethanExperiment4; Larger proportion cleave the eggs) Large num- 



larger proportion larvae than in 4; fair number ber good blastulae and 



blastulae gastrulae formed 



(Vesicle barely distinguish- 

 able in most) Similar to 

 Experiment 5 



(About one-third of the eggs (About one-half of the eggs 



maturing) Cleavage asj maturing) Larger pro- 



in Experiment 5; no lar- 1 portion of larvae than in 



vae found 5 



(Vesicle indistinct in most) (Like 6: one-third matur- 

 Numerous larvae formed;; ing) Cleavage rather 

 more favorable than Ex- more regular than in 



periment 6 



(Region of germinal vesicle 

 almost indistinguishable) 

 Larger proportion cleave 

 and more regularly than 

 in 7; next morning most 

 eggs in blastula or early 

 gastrula stage 



(Like 8) Most eggs form 

 vigorous gastrulae by next 

 morning; some reach Bi- 

 pinnaria stage later. Very 

 favorable culture 



6; fair proportion form 

 blastuls 



(One-third mature; first 

 polar spindle visible as 

 clear area at surface of 

 egg) A few blastulae 

 formed; less favorable 

 than 7 



(As in 8) Less favorable 

 than 8; no larvae 



(Vesicle quite invisible in (Like 8 and g) Unfavor- 

 practically all eggs) Less' able; eggs take irregular 



favorable than 9; till as 

 large proportion form vig- 

 orous larvae a few of which 

 reach early Bipinnaria 

 stage 



shapes; no larvae 



(Like 6) More favorable 

 than 6; large proportion 

 of good gastrulae formed 



(As before; first polar spin- 

 dle visible in about one- 

 half of the eggs) Large 

 proportion of eggs form 

 gastrulae as in 7; rather 

 more favorable 



(Like 8) Cleavage slower 

 and fewer larvae formed 

 than in 8; still very fa- 

 vorable 



(Like 8; polar bodies not 

 yet separated) Less fa- 

 vorable; cleavage more 

 irregular and few form 

 larvae 



