4i6 Ralph S. Lillie 



The following series will illustrate: 



TABLE VIII 



August 24, IQ07. Eggs were removed at 2:5S p-f- and after JO minutes in normal sea-water were transferred 

 to ^^7— KCN in sea-water. In this solution, after the intervals indicated, successive portions were 

 warmed to 55° for "JO seconds, and immediately transferred to normal sea-water. In each experiment 

 the eggs were allowed to settle and the sea-water was changed and this washing process was repeated 

 a second time 



Control warmed in normal sea-water: Three portions were warmed in normal sea-water (without 

 previous cyanide treatment) at respectively 30, 40 and 50 minutes after removal. All three formed 

 numerous active larvae; the conditions, however, were decidedly less favorable than with the cyanide- 

 treated eggs; most eggs died in early stages, development was slower, and the resulting larvae were less 

 active and normal than in the favorable cyanide cultures. 



Sperm-fertilized controls: Sperm was added to five successive portions at 35 m., 57 m., I h. 17 m., 

 I h. 50 m. and 3 h. 10 m. after removal; numerous active larvae were obtained in all but the last; on the 

 whole, the best sperm-culture was inferior to the best ■cyanide-culture and reached less advanced stages 

 of development. 



A second series on August 27 gave similar results though the 

 eggs were not so favorable. The result was, however, all the 

 more striking since the best cyanide cultures were found to give 

 a larger proportion of active normal larvae than were obtained 

 with sperm fertilization, even at the most favorable time. 



