422 



Ralph S. LiUie 



TABLE XI 



September 2, igoj. The eggs were left in sea-water for 45 minutes after removal; then transferred to .i^^-^ 

 KCN for one hour, warmed to 35° for "JO seconds in this solution, retransferred to ^^j, KCN at 

 normal temperature, and thence, after the designated intervals, transferred to normal sea-water which 

 was changed twice to remove all cyanide. 



Controls warmed in normal sea-water, 50 and 65 minutes respectively after removal, gave a fair num- 

 ber of blastulae after' 24 hours, of which a few were beginning to gastrulate. As compared with Experi- 

 ments I to 6 above, the larvae are fewer and in a less advanced stage of development. 



Of the sperm-fertilized controls, those warmed within I h. 30 m. after removal gave a large number 

 of normal active larvae. 



On examination, after 24 hours, of eggs left in the cyanide solution, all were found with membranes^ 

 round, clear, uncoagulated and uncleaved; many, however, showed little pseudopodia-like projections, 

 and frequently small portions of the surface-protoplasm had become detached from the egg. While 

 cleavage is impossible in the KCN solution, there appears nevertheless to have been some slight cyto- 

 plasmic activity in these eggs. 



In a third series similar conditions were found; in this series 

 the eggs were unfavorable and very few larvae resulted even in the 

 best sperm-fertilized control. A relatively small proportion of eggs 

 formed larvae in the best experiments; still, exposure to 2/00 KCN 



