From the Physiological Laboratory, Washington University Medical School, 1806 Locust Street, 

 Saint Louis, Missouri. 



FURTHER RESULTS OF TRANSPLANTATION OF 

 OVARIES IN CHICKENS^ 



BY 



C. C. GUTHRIE, M.D., PH.D. 



With Three Figures 



INTRODUCTION 



Numerous attempts have been made to transplant ovaries in 

 animals with the view of studying their function after the opera- 

 tion. In general, such attempts have been unsuccessful. 



In 1903 Prof. E. P. Lyon, then at the University of Chicago, 

 invited me to join him in studying ovarian transplantations in 

 bitches. Feeling confident that the operations would be unsuc- 

 cessful with the surgical facilities at our command, and knowing 

 of the high resistance of fowls to infection and to surgical shock, 

 I suggested the use of chickens.^ 



EXPERIMENTS 



We operated upon two adult hens but the results were unsatis- 

 factory. It was in the laying season (February, 1904) and we 

 attributed the operative results to the unfavorable condition of 

 the ovaries. We then determined on using young chickens but 

 before we could carry out this intention Professor Lyon assumed 

 the direction of another laboratory and was unable to continue 

 in the work. 



During the summer of 1904 (August 18) I exchanged the 

 ovaries between two black and two white leghorn pullets, weigh- 



' A preliminary report was made before the American Physiological Society in Washington City, May 

 7, 1907. (See Proceedings of the Society, American Journal of Physiology, July, 1907, vol. xix, pp. 

 xvi-xvii). Results of this work was also reported at the Seventh International Congress of Physiolo- 

 gists, Heidelberg, August 13-16, 1907. (See Archives Internationales de Physiologic, 1907, vol. v, 

 p. 108.) 



2 Since this paper was written I have learned that Dr. E. P. Lyon, even before my acquaintance with 

 him, planned a similar series of experiments on pigeons and even made some introductory experiments 

 on them. His reason for selecting the pigeon was that it has but a single functional ovary. 

 The Journal of Experimental Zoology vol. iv, no. 4 



