The Energy of Segmentation 307 



stages were foreseen, but the results obtained for the first two 

 stages showed that the volume after the first segmentation was 

 the same as before it; it could, therefore, be assumed to be con- 

 stant during the second and third and even subsequent stages, 

 especially since general observation makes no change manifest. 

 On the other hand, the increase of surface which was demonstrated 

 to have taken place was shown to be not of direct significance in 

 the computation made of the energy of segmentation. 



These measurements of diameters were made in Experiments 

 V and VI on eggs taken, of course, from the control. ^^ But it is 

 evident that the numerical results thus obtained are in any com- 

 plete computation to be combined with those obtained from the 

 use of the inhibiting solutions on eggs of the same lot. Accord- 

 ingly, it is the result of the complete computation from all the 

 necessary experimental data, as taken in Experiment VI, that is 

 presented below; and in connection therewith it may be remarked 

 that, as between method and numerical result, it is the former rather 

 than the latter that I would have regarded as the more worthy 

 of emphasis. If the question be raised as to the accuracy of the 

 numerical result, this can be estimated by considering the sources 

 and probable limits of error introduced both by the method of 

 compensating and by the fact that one is observing a "group" 

 of eggs and must adopt the expedient of taking averages, etc. 



OBSERVED AND COMPUTED RESULTS IN EXPERIMENT VI 



Data: Segmentations stopped, the first by 15 cc, § m. sugar 



sol. plus 16.75 P^^^ ^'5 ^^- ^- ^- ^^ ^3-5° ^-j ^^c- 



Now, it is well known that osmotic solutions in general follow 

 the law for gases; and it is held, too, that there is no dissociation 

 in a sugar solution. Accordingly the pressure of a mol. sugar sol. 

 at 0° C. is 22.4 atmospheres." 



^Miss Evis Berry kindly assisted me in the experiment in this way. 



''An atmosphere is that unit of pressure which is exerted by a column of mercury 76 cm. in height 

 of a density 13.596; this equals in C. G. S. terms 1013300 dynes, i.e., the pressure of such a column of 

 mercury per sq. cm. The egg has of course an area which is only a small fractional part of a square 

 centimeter. 



