344 ^- J- (joldfarb 



was left between the water and the cork, then the flask was hermet- 

 ically sealed. The following tables indicate in per cent the vol- 

 ume of water after boiling. These figures will serve in a general 

 way to indicate the amount of oxygen removed. 



TABLE 20a 

 No. of hydranths observed on the following days No. of stems 



2d 3d 4th 5th 6th 7th 8th 9th loth icxs 



1 Norm sea-water in 



open dish 40 18 8 7 8 11 13 8 6 



2 Norm sea-water in 



sealed vessel 14 13 12 3 5 6 5 o o 



3 to lO; from 97% to 77% 000000000 



There was absolutely no sign of regeneration in the eight sealed 

 flasks, Nos. 3 to 10 inclusive, from which the oxygen had been 

 removed from the sea-water and its reabsorption prevented. Flask 

 No. 2 which was also hermetically sealed, contained normal 

 sea-water, and regenerated fewer polyps than the open dish No. 

 I. Regeneration in the former ceased altogether after eight 

 days, while in the latter polyps continued to be produced. Regen- 

 eration in the hermetically sealed flask No. 2 was inhibited, 

 either because the supply of oxygen in the water was entirely 

 appropriated by the previously developed polyps or because of 

 the carbon dioxide produced by these polyps, or by both of these 

 causes acting together. 



Additional evidence of a very interesting nature was obtained 

 by filling wide mouthed jars about two-thirds full of oxygen — free 

 sea-water as above. There was, however, a layer of air over one 

 inch deep between the water and the cover, which was sealed air- 

 tight. 



TABLE 20b 



No. 



* Probably an error. 



