338 N. E. McINDOO 



does not come in direct contact with the chitin around the pores; 

 (2) as the sense fibers end in the neck of the funnel-Hke aperture 

 and do not extend into the mouth of the funnel, their peripheral 

 ends do not attain the level of the external surface of the chitin. 

 It thus seems impossible for these thick substances to run into 

 the mouths of the funnel-like apertures, where they must force 

 out the air in order to come in contact with the ends of the sense 

 fibers. 



To anesthetize the ends of the sense fibers with a finely pow- 

 dered anesthetic, either by blowing or by applying the powder 

 with a brush on the parts possessing the pores, is impossible for 

 two reasons: (1) The powder does not come in contact with 

 the chitin on account of the dense coat of hair; (2) the diameter 

 of the smallest particles of the powder is microscopically two or 

 three times that of the largest pore apertures. 



To anesthetize the ends of the sense fibers by using an anesthetic 

 dissolved in water, the following procedure was employed : With 

 a small pencil brush the pores on the legs and wings were cov- 

 ered with a one-fifth saturated solution of alypin, a synthetic 

 anesthetic. The bee was immediately put into a small observa- 

 tion case and the latter was held in the current of an electric 

 fan for five minutes. By this time the bee was again dry and 

 appeared normal in most respects, but it was extremely restless 

 and did not eat candy until several minutes afterwards. Since 

 the bee did not become perfectly quiet until two or three hours 

 afterwards, a reaction time in seconds could not be obtained while 

 the anesthetization was effective. In view of the fact that the 

 rate of walking of unmutilated bees may be increased by using 

 odors, this anesthetized bee was, tested with oil of peppermint 

 to determine whether odors cause its rate of movement to be 

 increased. The word excited is used to describe the action of 

 the bee when it quickly moved from over the odor and vigorously 

 vibrated its wings. In all, 10 workers were used, with the fol- 

 lowing results: 



Two workers: During the first 10 minutes their slow rate of 

 walking was not increased; after 15 minutes one was easily ex- 

 cited; after 30 minutes the other could not be excited and in- 



