STUDIES OF FERTILIZATION 



VI. THE MECHANISM OF FERTILIZATION IN ARBACIA 



FRANK R. LILLIE 



Marine Biological Laboratory, Wood's Hole, Mass., and 

 Hull Zodlogical Laboratory, University of Chicago 



ONE FIGURE 



CONTENTS 



I. Introduction 524 



II. The spermophile side-chain 525 



1. A quantitative method of studying the production of fertilizin by 



ova 525 



2. The quantity of fertilizin produced by ova of Arbacia 528 



a. By a single washing 628 



b. Comparison of ripe and unripe eggs * 530 



c. Fertilizin produced in a series of washings: role of the jelly. . . . 532 



3. The binding of fertilizin by sperm 535 



4. Separateness of heteroagglutinating substances in egg-secretions .. . 541 



5. Some special properties of fertilizin 543 



6. Anti-fertilizin 544 



7. The necessity of fertilizin for fertilization 549 



a. Fertilization of washed eggs 549 



b. Cessation of fei^ilizin production after fertilization 553 



c. Cessation of fertilizin production after formation of membranes 



by butyric acid 557 



8. Summary of Part II 561 



III. The ovophile side-chain 562 



Introduction 562 



1. The inhibiting effect of blood of the sea-urchin upon fertilization of 



eggs of the same species 563 



2. Mode of action of the inhibitor 570 



3. Summary of Part III 577 



IV. General discussion 577 



1. The mechanism of fertilization 578 



2. The two phases of fertilization 582 



3. The lysin theory of fertilization .• 584 



4. The theory of parthenogenesis 587 



V. Literature cited 589 



523 



THE JOURNAL OF EXPERIMENTAL ZOOLOGY, VOL. 16, NO. 4 



